The development of DFAST 2.0 signals a shift in the battery narrative. For decades, the industry has been trying to force lithium to be better, squeezing incremental improvements out of a chemistry that is becoming increasingly expensive and dangerous.
Magnesium, empowered by DFAST 2.0 electrolytes, offers a pathway to a "post-lithium" world. The technology promises batteries that are:
Even with release 7, some issues persist:
Issue 1: Protein truncation warnings for pangenome contigs.
--min-contig-len 1000 (default 500) to avoid annotating tiny fragments.Issue 2: Slow DIAMOND search for very large genomes (>12 Mbp).
--evalue 1e-6 to sacrifice slight sensitivity for speed.Issue 3: Error: "Cannot locate taxonomy for species."
--taxid 562 (for E. coli) instead of relying on the species name string.Annotation is only as good as its database. With dfast 2.0 7, the default protein clusters were synced with:
This database refresh alone reduced hypothetical protein assignments by ~8% for common Enterobacteriaceae genomes.
Previous DFAST 2.0 versions often mis-annotated small plasmid open reading frames (ORFs) as contaminants or truncated copies. Version 7 implements a plasmid-aware heuristics system that cross-references the Plasmid RefSeq database before discarding short ORFs. Resulting in 12-15% fewer false negatives for small plasmid genes.
The development of DFAST 2.0 signals a shift in the battery narrative. For decades, the industry has been trying to force lithium to be better, squeezing incremental improvements out of a chemistry that is becoming increasingly expensive and dangerous.
Magnesium, empowered by DFAST 2.0 electrolytes, offers a pathway to a "post-lithium" world. The technology promises batteries that are:
Even with release 7, some issues persist: dfast 2.0 7
Issue 1: Protein truncation warnings for pangenome contigs.
--min-contig-len 1000 (default 500) to avoid annotating tiny fragments.Issue 2: Slow DIAMOND search for very large genomes (>12 Mbp). dfast 2
--evalue 1e-6 to sacrifice slight sensitivity for speed.Issue 3: Error: "Cannot locate taxonomy for species."
--taxid 562 (for E. coli) instead of relying on the species name string.Annotation is only as good as its database. With dfast 2.0 7, the default protein clusters were synced with: Common Pitfalls in DFAST 2
This database refresh alone reduced hypothetical protein assignments by ~8% for common Enterobacteriaceae genomes.
Previous DFAST 2.0 versions often mis-annotated small plasmid open reading frames (ORFs) as contaminants or truncated copies. Version 7 implements a plasmid-aware heuristics system that cross-references the Plasmid RefSeq database before discarding short ORFs. Resulting in 12-15% fewer false negatives for small plasmid genes.